![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K1899006
Designed by: iGEM2016_HKUST Group: iGEM16_Hong_Kong_HKUST (2016-10-10)
J23101-B0032-lacl-B1006- phlFp-GFP
This construct aims at investigating any interference caused by Lacl repressor on promoter phlFp.
Results
The fold change between construct A (pSB3K3-BBa_E0240-phlFp) and negative control (pSB3K3-BBa_E0240), and that of construct C (pSB3K3-BBa_J23101-B0032-C0012-B1006- phlFp -E0240) were both approximately 13.2. Since their RFU are similar, it indicated that there is no cross-talk between lacp and phlFp (BBa_K1899004).
![](/wiki/images/4/42/IGEM2016_HKUST_phlFK1899006.png)
Fig 1. Comparison on fluorescence expression levels of construct A (BBa_phlFp-E0240) and construct C (BBa_J23101-B0032-C0012-B1006- phlFp -E0240). Negative control represents BBa_E0240. Characterization was done using E. coli strain JW0336. Cells were first precultured overnight and were subcultured to mid-log phase where GFP emission measurements were made using an EnVision® multilabel reader. This result was obtained by combining 3 characterization data obtained in 3 different days. Error bar present SD from 3 biological replicates.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1209
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2007
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Categories
Parameters
//chassis/prokaryote/ecoli
None |